Chromosomal destabilization during gene amplification.

Gene amplification during myogenic differentiation

Gene amplifications are mostly an attribute of tumor cells and drug resistant cells. Recently, we provided evidence for gene amplifications during differentiation of human and mouse neural progenitor cells. Here, we report gene amplifications in differentiating mouse myoblasts (C2C12 cells) covering a period of 7 days including pre-fusion, fusion and post-fusion stages. After differentiation in...

Non-Allelic Homologous Recombination Gene Amplification Chromosomal Rearrangements

Chromosomal and gene amplification in diffuse large B-cell lymphoma.

Chromosomal translocations leading to deregulation of specific oncogenes characterize approximately 50% of cases of diffuse large B-cell lymphomas (DLBL). To characterize additional genetic features that may be of value in delineating the clinical characteristics of DLBL, we studied a panel of 96 cases at diagnosis consecutively ascertained at the Memorial Sloan-Kettering Cancer Center (MSKCC) ...

Adaptive Amplification An Inducible Chromosomal Instability Mechanism

Adaptive mutation is an induced response to environmental stress in which mutation rates rise, producing permanent genetic changes that can adapt cells to stress. This contrasts with neo-Darwinian views of genetic change rates blind to environmental conditions. DNA amplification is a flexible, reversible genomic change that has long been postulated to be adaptive. We report the discovery of ada...

Chromosomal changes without DNA overproduction in hydroxyurea-treated mammalian cells: implications for gene amplification.

It has been reported that a 6-h incubation of early S-phase Chinese hamster cells with hydroxyurea promotes DNA overproduction, i.e., replication of DNA a second time within a single cell cycle, and that this could be the basis for gene amplification in drug-treated mammalian cells. When we incubated methotrexate-resistant Chinese hamster cells that were approximately 2 h into the S phase with ...